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Elements and Performance Criteria

  1. Classify organisms
  2. Obtain biological samples
  3. Review work request to identify required samples and the procedures, materials and equipment involved
  4. Identify hazards and workplace control measures associated with collecting the sample, preparation methods, reagents and equipment
  5. Assemble required materials, reagents and equipment and check they are fit for purpose
  6. Collect samples in accordance with legislative requirements, codes of practice and workplace procedures
  7. Log samples using workplace procedures
  8. Record sample description, compare with specification and note and report discrepancies
  9. Ensure traceability from receipt of sample to reporting of results
  10. Prepare samples and grow cultures
  11. Obtain samples for microbiological analysis
  12. Identify contamination pathways for both samples and media
  13. Perform simple chemical tests to identify morphology of environmentally significant bacteria
  14. Use aseptic techniques to manipulate samples and bacterial cultures
  15. Use standard techniques to grow cultures of environmentally significant microorganisms
  16. Use disinfection and sterilisation to control the growth of microorganisms
  17. Prepare microscope for use
  18. Check that all microscope components are clean and fit for purpose
  19. Mount sample in accordance with specified method
  20. Adjust settings and alignment of optical and mechanical components to optimise performance
  21. Perform microscopic examination in accordance with test methods
  22. Measure organisms and structures
  23. Enumerate microorganisms in environmental samples
  24. Record all observations and measurements used to determine classifications
  25. Relate cell structures and their functions to environmental adaptation
  26. Associate biological functions with cell structures
  27. Associate cell physiology and enzyme cycles to environmental adaptation
  28. Identify important biochemical pathways associated with environmentally significant organisms
  29. Maintain a safe work environment
  30. Handle all samples and equipment in accordance with workplace safety procedures
  31. Clean up spills using techniques to protect personnel, work area and environment
  32. Minimise generation of waste and environmental impacts
  33. Collect and dispose of all wastes safely
  34. Report hazards and incidents to designated personnel using workplace procedures
  35. Maintain workplace records
  36. Maintain confidentiality and security of workplace information and data